Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design

Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design.

The effects of 5 factors (template DNA, Mg(2+), dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymor-phism (SCoT)-PCR system of Dactylis glomerata L., using an orthogo-nal design L16 (4(5)). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction volume contained 3.0 mM Mg(2+), 0.2 mM...

Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi.

Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L16 (45) design was employed to investigate the effects of five factors (Mg2+, dNTPs, Taq DNA polymerase, primer, and template DNA) on the ...

Genetic Diversity and Association of EST-SSR and SCoT Markers with Rust Traits in Orchardgrass (Dactylis glomerata L.).

Orchardgrass (Dactylis glomerata L.), is a well-known perennial forage species; however, rust diseases have caused a noticeable reduction in the quality and production of orchardgrass. In this study, genetic diversity was assessed and the marker-trait associations for rust were examined using 18 EST-SSR and 21 SCoT markers in 75 orchardgrass accessions. A high level of genetic diversity was det...

Fungal Diversity The endophytic mycobiota of the grass Dactylis glomerata

Fungal endophytes were isolated from asymptomatic and symptomatic plants of Dactylis glomerata sampled in different ecosystems in Spain. Fungi were identified using morphological, as well as molecular methods based on internal transcribed spacer (ITS) and ribosomal DNA sequencing. Molecular data provided a framework for identification and assessing the phylogenetic position of isolates. One hun...

Increased Meiotic Irregularity Accompanying Inbreeding in Dactylis Glomerata L.